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Feature and Advantages:
?Hardly getting aff ected by the molecular size of HA
?Measurable the HA concentrations accurately in blood serum, plazma and supernatant of which the molecular weight is above 7.4 kDa.
?Measuring range of HA concentration is between 3.13 - 200 ng/mL.
Measurement principle: A sample containing HA and Biotin-HABP are added to an HA-coated plate. HRP-Avidin is then added, and the HA binding Biotin-HABP is detected by chromophoric substrate (Competition Method)
Measuring Range
calibration curve: The measuring range of HA using Hyaluronan Quantification Kit is 3.13~200 ng/mL.
Product Data: Specificity
The reactivity with various molecular weights of HA (HA with molecular weights of from 2.8 to 2367 kDa were compared)
The reactivity with various molecular weights of HA was evaluated using Hyaluronan Quantification Kit . When using HA with molecular weights of from 2.8 to 2367 kDa, this kit reacted with molecular weights of over 7.4 kDa HA in a similar manner. HA with a molecular weight of 2.8 kDa showed a slightly weaker reactivity.
The cross-reactivity with various kinds of glycosaminoglycans (GAGs) was evaluated using Hyaluronan Quantification Kit. This kit did not show the cross-reactivity with chondroitin sulfate-A (CS-A), CS-C, CS-D, CS-E, heparan sulfate (HS), heparin (Hep), keratan sulfate (KS) and keratan polysulfate (KPS) at the concentrations of up to 1000 ng/mL. A weak reactivity was shown in high concentration of CS-B (dermatan sulfate).
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