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Vector Laboratories presents

NEW ImmPRESS Duet Double Staining Polymer Kits: Save time and streamline your double staining IHC workflow!

This kit enables fast, well-defined localization and visualization of two different target antigens on the same tissue section.

With a unique ImmPRESS™ micropolymer chemistry, highly-active HRP and AP enzyme polymers  are conjugated to anti-mouse IgG and anti-rabbit IgG affinity-purified (highly cross-adsorbed).

These reagents ready-to-use and detect mouse and rabbit primary antibodies, with a time-saving, simplified, one-step double label detection protocol.

The ImmPRESS™ Duet Double Staining Polymer Kit is intended for use on non-rodent tissue specimens. Reliable and reproducible results are obtained on tissue sections where the two target antigens do not over-lap (co-localize) in the same structure of the same cell, but rather are expressed in different cell compartments or different cell types.

Kit (sufficient for 150 sections) includes:

1) 15 ml BLOXALL™ Blocking Solution--> abolishes all endogenous peroxidase and alkaline phosphatase activity in a single 10 minute incubation

2) 15 ml 2.5% Normal Serum--> blocks all non-specific binding of detection reagents to ensure crisp, specific staining

3) 15 ml ImmPRESS™ Duet HRP/AP Polymer Reagent (mixture of Anti-Rabbit IgG and Anti-Mouse IgG)

4) ImmPACT™ DAB EqV Substrate (HRP, brown) --> for maximum sensitivity and staining contrast between target antigens

5) ImmPACT™ Vector® Red Substrate (AP, magenta)-->for maximum sensitivity and staining contrast between target antigens


Colon Carcinoma (paraffin): ImmPRESS Duet Kit (MP-7724) used to detect mouse anti-CD34 (ImmPACT DAB, brown) and rabbit anti-Ki67 (ImmPACT Vector® Red, magenta) antibodies.

ImmPRESS Duet Double Staining HRP/AP Polymer Kit (anti-rabbit IgG-brown, anti-mouse IgG-red)

Catalog Number: MP-7714
 
 

 

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