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Anti Iba1
, Rabbit Calcium ions are known to be one of the most important signal mediators in all cells including central nervous system (CNS) cells. Calcium ions exert their signaling activity through association with various calcium binding proteins, many of which are classified into a large protein family, the EF hand protein family.Iba1
is a 17-kDa EF hand protein that is specifically expressed in macrophages/ microglia and is pregulated during the activation of these cells.Wako supplies rabbit polyclonal antibodies were raised against a synthetic peptide corresponding to the Iba1 carboxy-terminal sequence, which was conserved among human, rat and mouse Iba1 protein sequences. These antibodies are specifically reactive to microglia/macrophages, are appropriate for immuno-double staining of brain tissues and cell culture in combination with monoclonal antibody to GFAP, which specifically reacts to astrocyte.
Specificity: Specific to human, mouse and rat microglia and macrophages, but not cross-reactive with neurons and astrocytes.
For Immunocytochemistry
Wako Cat. No. 019-19741 50µgFor Western Blotting
Wako Cat. No. 016-20001 50µgImages of a double stain and a phase contrast of the same field in rat primary mixed culture cells |
Western blotting
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Related Products
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- Imai, Y., Ibata, I., Ito, D., Ohsawa., K. and Kohsaka, S.: Biochem. Biophys. Res. Commun., 224, 855(1996).
- Ito, D., Imai, Y., Ohsawa, K., Nakajima, K., Fukuuchi, Y. and Kohsaka, S.: Brain Res. Mol. Brain Res., 57, 1(1998).
- Ohsawa, K., Imai, Y., Kanazawa, H., Sasaki, Y. and Kohsaka, S.: J. Cell Sci ., 113, 3073 (2000).
- Sasaki, Y., Ohsawa, K., Kanazawa, H., Kohsaka, S. and Imai, Y.: Biochem. Biophys. Res. Commun ., 286, 292(2001).
- Kanazawa, H., Ohsawa, K., Sasaki, Y., Kohsaka, S. and Imai, Y.: J. Biol. Chem., 277, 20026 (2002).
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