Gentile Cliente,

grazie alla www.revmab.com , una biotech californiana specializzata nella produzione di Rabbit & Goat Monoclonals, siamo lieti di potervi proporre una valida alternativa all' anticorpo clone VE1 della SpringBio specifico per la forma mutata del BRAF (V600E):

BRAF V600E Rabbit Monoclonal Antibody, Clone RM8, BRAF(V600E), Cat# 31-1042-00

http://www.revmab.com/index.php/product/rabbit-monoclonal-braf-v600e-antibody/

"We have validated our RM8 clone in melanoma and thyroid carcinoma. Feedback from customers so far has been that the RM8 clone is comparable to that of VE1 with higher sensitivity"

L' anticorpo è disponibile in due formati da 25 ug e 100ug.      

Esempi di applicazioni:

Immunohistochemical staining of formalin fixed and
paraffin embedded melanoma tissue sections.

Immunohistochemical staining of formalin fixed and
paraffin embedded Thyroid Carcinoma tissue. Allele
specific PCR validated to be Negative for BRAF V600E.

Sandwich ELISA performed on the lysates of 293T
cells overexpressing BRAF V600E or BRAF wild type
His-tag proteins, shows the specificity of RM8 to the
V600E mutation. His-tag fusion proteins were captured
by an Anti-His-Tag antibody.

Western Blot analysis of cell lysates prepared from cell
lines expressing endogenous mutant (V600E) BRAF or
wild type (WT) protein.