Vector Laboratories presents:
Vector® TrueVIEW™ Autofluorescence Quenching Kit. Cat# SP-8400
Tissue autofluorescence often occurs with aldehyde fixation or from inherent native tissue components (collagen, elastin, and red blood cells). The extent and intensity of autofluorescence background frequently makes it difficult or impossible to distinguish specific signals in immunofluorescence applications.
Current methods for reducing autofluorescence primarily include “home brew” concoctions such as sodium borohydride and other ink-based products. These methods are essentially ineffective against aldehyde induced autofluorescence.
The Vector® TrueVIEW™ Autofluorescence Quenching Kit provides a novel way to diminish unwanted autofluorescence from non-lipofuscin sources and dramatically improve signal-to-noise ratio. The quenching action of the kit reagents provides a clear, unambiguous, “true view” localization of the target antigen. Vector® TrueVIEW™ reagent binds to hydrophilic compounds and effectively quenches endogenous autofluorescence.
Features:
- Removes unwanted autofluorescence in tissue sections due to aldehyde fixation, red blood cells, and structural elements such as collagen and elastin
- Improves overall signal-to-noise in most immunofluorescence assays
- Effective on problematic tissues such as kidney and spleen
- Easy-to-use, one-step method
- Compatible with a wide selection of fluorophores
- Compatible with standard epifluorescence and confocal laser microscopes
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Spleen (FFPE), antigen retrieved with Antigen Unmasking Solution, stained using Mouse Anti-CD20 (red) and Rabbit Anti-Ki67 (green), followed by VectaFluor™ Duet Kit, and mounted in VECTASHIELD® HardSet™ Mounting Medium with DAPI. Left: No TrueVIEW™ Quencher treatment - note interfereing autofluorescence. Right: Treated with TrueVIEW™ Autofluorescence Quencher.
No TrueVIEW™ Quencher => 20x objective, red channel exposed 200ms, green channel 200ms
With TrueVIEW™ Quencher => 20x objective, red channel exposed 200ms, green channel exposed 800ms |
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Pancreas (FFPE), antigen retrieved with Antigen Unmasking Solution, stained with Guinea Pig x Insulin (green) and Mouse x END (red), followed by FL-Goat x Guinea Pig IgG + Dylight® 594-Horse x Mouse IgG. Mounted in VECTASHIELD® HardSet™ Mounting Medium with DAPI.
No TrueVIEW™ Quencher => 20x objective, red channel exposed 600ms, green channel 600ms
With TrueVIEW™ Quencher => 20x objective, red channel exposed 600ms, green channel exposed 600ms |
More Info
Protocol |