20% OFF on High Sensitivity ELISA KIT for measuring UV-induced DNA damage campaign
From December 1st ,2017 to February 28th, 2018 only with promo Code: 1079
TDM-2 is widely cited in the literature and is considered to be the gold standard antibody for CPD detection and quantification.
Cosmo Bio's CPD ELISA Kit is the first and only commercially available ELISA kit using TDM-2. All kits components are optimized for high sensitivity CPD detection from cultured cells or skin epidermis.
ASSAY PRINCIPLE
The format of this assay is direct ELISA with colorimetric detection. In brief, genomic DNA purified from UV-damaged cells is heat denatured and applied to microtiter wells pre-coated with protamine sulfate. CPD specific monoclonal antibody clone TDM-2 (Cosmo Bio Cat. No. CAC-NM-DND-001) is then added to each well for thirty minutes and unbound antibody is removed by rinsing. The amount of TDM-2 antibody remaining in each well is then measured by sequential treatment of wells with biotinylated 2nd antibody, streptavidin-peroxidase, and o-Phenylenediamine (OPD). The reaction between peroxidase, H2O2 and OPD produces a yellow orange color, the strength of which is generally proportional to the amount of TDM-2 antibody remaining bound to the plate. The color development reaction is stopped and the absorbance of each well at 492 nm is measured with a spectrophotometer.
REACTIVITY
UV-induced CPDs in DNA measured by ELISA
Formation and repair of UV-induced CPDs in human cells measured by ELISA
UVC radiation induces CPDs in DNA of HeLa cells in dose-dependent manner. The initial level of CPDs induced by 10 J/m2 of UVC gradually decreases over time as CPDs are repaired, indicating the capacity of nucleotide excision repair in HeLa cells.
Product Name Cat# Quantity High Sensitivity CPD ELISA kit Ver.2 CSR-NM-MA-K003 1KIT CAC-NM-DND-001 1VIAL CSR-NM-MA-R010 5*10UG PROTAMINE SULFATE COATED ELISA PLATE 96 CSR-NM-MA-P001 1PLATE PROTAMINE SULFATE COATED ELISA PLATE 96 x 5 CSR-NM-MA-P002 1*5PLATE PROTAMINE SULFATE COATED ELISA PLATE 96 x 10 CSR-NM-MA-P003 1*10PLATE High Sensitivity 6-4PP/ (6-4) photoproduct ELISA kit CSR-NM-MA-K002 1KIT
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