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20% OFF on High Sensitivity ELISA KIT for measuring UV-induced DNA damage campaign

From December 1st ,2017  to February 28th, 2018 only with promo Code: 1079

 

 

Cosmo Bio's new Cyclobutane Pyrimidine Dimer (CPD) ELISA Kit uses the highly sensitive and specific monoclonal anti-CPD antibody clone TDM-2, established by Mori et al.

TDM-2 is widely cited in the literature and is considered to be the gold standard antibody for CPD detection and quantification.

Cosmo Bio's CPD ELISA Kit is the first and only commercially available ELISA kit using TDM-2. All kits components are optimized for high sensitivity CPD detection from cultured cells or skin epidermis.

ASSAY PRINCIPLE

The format of this assay is direct ELISA with colorimetric detection. In brief, genomic DNA purified from UV-damaged cells is heat denatured and applied to microtiter wells pre-coated with protamine sulfate. CPD specific monoclonal antibody clone TDM-2 (Cosmo Bio Cat. No. CAC-NM-DND-001) is then added to each well for thirty minutes and unbound antibody is removed by rinsing. The amount of TDM-2 antibody remaining in each well is then measured by sequential treatment of wells with biotinylated 2nd antibody, streptavidin-peroxidase, and o-Phenylenediamine (OPD). The reaction between peroxidase, H2O2 and OPD produces a yellow orange color, the strength of which is generally proportional to the amount of TDM-2 antibody remaining bound to the plate. The color development reaction is stopped and the absorbance of each well at 492 nm is measured with a spectrophotometer.

REACTIVITY

EXAMPLE OF RESULTS


UV-induced CPDs in DNA measured by ELISA


Formation and repair of UV-induced CPDs in human cells measured by ELISA
UVC radiation induces CPDs in DNA of HeLa cells in dose-dependent manner. The initial level of CPDs induced by 10 J/m2 of UVC gradually decreases over time as CPDs are repaired, indicating the capacity of nucleotide excision repair in HeLa cells.

Product Name

Cat#

Quantity

High Sensitivity CPD ELISA kit Ver.2

 

CSR-NM-MA-K003

1KIT

Anti CPDs

 

CAC-NM-DND-001

1VIAL

UVC-irradiated DNA samples

 

CSR-NM-MA-R010

5*10UG

PROTAMINE SULFATE COATED ELISA PLATE 96

 

CSR-NM-MA-P001

1PLATE

PROTAMINE SULFATE COATED ELISA PLATE 96 x 5

 

CSR-NM-MA-P002

1*5PLATE

PROTAMINE SULFATE COATED ELISA PLATE 96 x 10

 

CSR-NM-MA-P003

1*10PLATE

High Sensitivity 6-4PP/ (6-4) photoproduct ELISA kit

 

CSR-NM-MA-K002

1KIT

 

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