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Lung tissue from a resting adult mouse stained with Fluorescein-labeled Aleuria aurantia lectin (AAL; green), and biotinylated Concanavalin A (Con A) followed by DyLight® 549 Streptavidin (red). Image courtesy of Dr. Brian A Cobb, Case Western University, School of Medicine. |
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Elucidating carbohydrate structures present in nature can be as challenging as it is fascinating. Greater understanding of lectin binding specificities can help determine details of glycan structure and help address related questions of function.
The National Center for Functional Glycomics (NCFG), with the support of the NIH Common Fund for Glycosciences, is screening a selection of Vector Labs's lectins. Using the Consortium for Functional Glycomics (CFG) glycan arrays, as well as a custom-printed array with a limited number of positive glycans for the lectins, the NCFG analyzes the glycan specificity of each of our lectin lots, and provides these detailed lectin-glycan binding data on the NCFG website. The goal of this NCFG project is to support rigorous and reproducible glycobiology experiments.
This data provide uniquely valuable insights into glycan structures on the glycoproteins that interact with each lectin:
- Binding specificity of each lectin, for hundreds of glycan structures in the array, as well as a 'yes/no' binding interaction on the focused QA/QC array
- Data tabulated and conveniently summarized as a binding array, with peak height indicating degree of binding for each glycan
- Specific and detailed data on carbohydrate structure
- Lot-specific data - for confidence in the reproducibility of your binding data ( to be expanded to include new lots and biotinylated and fluorescent forms of the lectins)
- Confocal microscopy images of up to 4 tissues stained with lectins (in collaboration with Dr. Brian Cobb, Case Western University, School of Medicine)
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