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Amicon® Pro Purification System

Purify, exchange buffer and concentrate in one device

The Amicon Pro centrifugal protein purification system, which combines an affinity-based spin column with an Amicon Ultra 0.5 mL filter, enables you to purify, concentration, and exchange the buffer of your protein sample, all in the same, versatile device.

No need to transfer the sample, no risk of protein loss.

How Amicon® Pro Purification Works

The device combines affinity-based spin column purification with downstream sample concentration and buffer exchange. Featuring a large reservoir that accommodates a range of sample volumes, the device reduces the need for multiple centrifugation steps. Simply attach the included Amicon® Ultra filter for simultaneous elution, concentration and highly efficient diafiltration (>99%) in a single spin.

Amicon® Pro Application

 

Protocol Steps

Maximum Capacity

Benefits3

Purification

With buffer exchange and/or concentration
  • Bind
  • Clear and Wash
  • Elute/Concentrate
    +/- Buffer Exchange 

= 200 µL packed resin1
  • Speed
  • No sample transfer - No loss
  • Improved yield 

Purification only
  • Bind
  • Clear and Wash
  • Elute 

= 1000 µL packed resin2
  • Range of sample volumes can be processed
  • Single Elution - No fractions 

Diafiltration
  • Buffer Exchange +/- Concentrate 

Not Applicable
  • Speed
  • Improved activity of purified protein 

Antibody labeling
  • Buffer Exchange
  • Labeling Reaction
  • Wash and Concentrate
    +/- Buffer Exchange 

variable antibody input3
  • Speed
  • No sample transfer - No loss
  • Improved yield
  • Read application note 

Depletion or Enrichment
  • Bind
  • Deplete/Concentrate
  • +/- Wash/Concentrate
    +/- Buffer Exchange 

= 200 µL packed resin1
  • Speed
  • No sample transfer - No loss 

Read our protocols and learn how the Amicon Pro system can simplify your protein preparation!

Download PDF

 

Richiedi un'offerta

DBA-ITALIA Srl
Via Umbria 10
20090 Segrate (Milano)

Tel. +39 02-26922300
Fax +39 02-26926058
Fax +39 02-26923535




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