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A useful tool for any mouse-on-mouse application | |
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The specific detection of mouse primary antibodies on mouse tissue can be difficult due to the presence of endogenous immunoglobulins. Conventional anti-mouse detection systems cannot distinguish between the mouse primary antibody and the endogenous mouse immunoglobulins, which leads to high background noise.
M.O.M.® (mouse on mouse) technology eliminates this problem by significantly reducing undesired binding. Excellent staining results for a once difficult application have now become routine with M.O.M. kits.
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Mouse kidney sections stained with anti-smooth muscle action mouse primary antibody.
Left: Confusing background seen using standard anti-mouse IHC-AP detection.
Right: Background eliminated leaving only specific staining using M.O.M. IHC-AP detection.
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Frozen mouse intestine sections double labeled (fluorescence) using two mouse primary antibodies against peripherin and desmin.
Left: Confusing background and signal mixing using standard anti-mouse IF detection.
Right: Clean background and specific signal using M.O.M. IF reagents and procedure. | | |
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Why researchers look to Vector Labs for mouse on mouse immunodetection |
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Significant reduction of endogenous mouse Ig staining | |
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Clear, crisp specific staining | |
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Detect several mouse primary antibodies on the same tissue section | |
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No tedious calculations or antibody prebinding steps required | |
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