We currently have two main types of tests for COVID-19, which are the molecular test and the serological test. Unlike the molecular test, the serological test detects the antibodies in the blood sample, usually obtained through a finger prick, giving better patient compliance. Besides, the serological test can identify those that were infected and have recovered, which gives a broader picture of COVID-19 infection spread.
Recent work was shared online by a team from the Southern University of Science and Technology. They developed a serological test for the diagnosis of SARS-Cov-2 using the chemiluminescence immunoassay method, which was explained in detail previously (Fig.1). The recombinant nucleocapsid protein of SARS-CoV-2 was successfully expressed from E. coli. and then immobilized to the magnetic beads to capture the IgM and IgG induced by SARS-CoV-2.
Fig 1. Detections and measurements of the SARS-CoV-2 IgM and IgG antibody in healthy people, tuberculosis patients and SARS-CoV-2 confirmed patients using the chemiluminescence immunoassay method (A and B). The average results were expressed as mean ± SEM of all individuals. Receiver Operating Characteristic curves for IgM (C) and IgG(D) were obtained based on the RLU for the SARS-CoV-2 patient group and the control group consisting of healthy cohorts and tuberculosis patients.
Reference: Dachuan Lin et al. Evaluations of serological test in the diagnosis of 2019 novel coronavirus (SARS-CoV-2) infections during the COVID-19 outbreak.
ACROBiosystems has developed different types of N protein. The binding activity of N protein with anti-N protein antibody was verified by ELISA, with a high sensitivity of 0.02 ng/mL.
Product:SARS-CoV-2 (COVID-19) Nucleocapsid protein, His Tag (C-terminus) Cat. No.:NUN-C5227
The sensitivity of N protein (His tag at the C-terminus) with anti-N protein antibody was 0.02 ng/mL as verified by ELISA. Immobilized SARS-CoV-2 Nucleocapsid protein, His Tag (Cat. No. NUN-C5227) at 2 µg/mL (100 µL/well) can bind SARS-CoV-2 NP Antibody, Rabbit MAb (CLN27) with a linear range of 0.02-0.6 ng/mL.
Product:SARS-CoV-2 (COVID-19) Nucleocapsid protein, His Tag (N-terminus) Cat. No.:NUN-C51H9
The sensitivity of N protein (His tag at the N-terminus) with anti-N protein antibody was 0.02 ng/mL as verified by ELISA. Immobilized SARS-CoV-2 Nucleocapsid protein, His Tag (Cat. No. NUN-C51H9) at 2 µg/mL (100 µL/well) can bind SARS-CoV-2 NP Antibody, Rabbit MAb (CLN27) with a linear range of 0.02-0.3 ng/mL.
The sensitivity of biotinylated N protein with anti-N protein antibody was 0.02 ng/mL as verified by ELISA. Immobilized SARS-CoV-2 NP Antibody, Rabbit MAb (CLN27) at 2 µg/mL (100 µL/well) can bind Biotinylated SARS-CoV-2 Nucleocapsid protein, His,Avitag (Cat. No. NUN-C81Q6) with a linear range of 0.4-3 ng/mL.
