:: DBA Newsletter ::

TdB Labs is specialised in the development and manufacturing of dextran derivatives and other polysaccharides.

They offer fluorescent derivatives with FITC, TRITC, ATTO™-dyes and their proprietary Antonia Red™-dye.

Their non-fluorescent derivatives include e.g. different variants of dextran sulfate, including dextran sulfate sodium (DSS) for colitis.

TdB Labs stands at the forefront, offering an exceptional range of Blue dextran products,

renowned for their quality and adaptability across a spectrum of molecular weights, from 5 kDa to 2000 kDa.

Their Blue dextran is synthesized from specific dextran fractions by reacting with Cibacron blue F3GA,

followed by a strict purification process to remove any unbound dye from the product, thereby ensuring its utmost quality.

Their Blue dextran’s formulation guarantees over six years of stability when stored in dry, well-sealed containers at ambient temperature.

Further, its solubility in water enhances its usability across various applications, ensuring ease of use for researchers.

The study of blood-brain barrier (BBB) permeability is crucial for several scientific and medical applications, including:

Drug Delivery: The BBB poses a significant challenge in delivering therapeutic agents to the brain.

Research into BBB permeability can aid in designing drugs that can effectively cross the barrier.

Neurological Disorders: Many neurological diseases, such as Alzheimer’s,

multiple sclerosis, and stroke, are associated with BBB dysfunction.

Understanding BBB permeability can help in diagnosing and developing treatments for these conditions.

Toxicology: Assessing how various substances affect BBB integrity is crucial

for evaluating the safety of new drugs and chemicals.

Several methods are employed to study BBB permeability, with dyes like blue dextran and Evans Blue (EB) being among the most commonly used.

While both blue dextran and Evans Blue (EB) are valuable tools for studying BBB integrity, blue dextran offers distinct advantages.

Its high specificity and low toxicity make it a safer and more reliable choice for in vivo studies.

Unlike EB, which can bind non-specifically and pose toxicity risks, blue dextran provides clear, accurate results without false positives.

1- Blue Dextran: Blue dextran is a high molecular weight polysaccharide that does not normally cross the BBB.

It is used to assess the integrity of the barrier by tracking its presence in brain tissue.

Advantages:

High Specificity: Blue dextran, a high molecular weight polysaccharide, does not normally cross the BBB,

making it a reliable marker for assessing barrier integrity.

Low Toxicity: It is generally considered safe for in vivo studies.

Limitations:

Sensitivity: Blue dextran may not be as sensitive as other markers for detecting minor disruptions in the BBB.

Visualization: While it can be visualized using various imaging techniques, it may not be as straightforward as some other dyes.

DBA-ITALIA Srl
Via Umbria 10
20054 Segrate (Milano)

Tel. +39 02-26922300

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