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Texas Red® Peptide Labeling Drawbacks & Alternatives


Sulforhodamine 101 acid chloride (also called Texas Red®) is the most popular labeling reagent of sulfonyl chlorides. Unfortunately it is quite unstable in water, especially at the higher pH required for reactions with aliphatic amines, and reacts indiscriminately. In addition, the labeling efficiency is extremely low compared to succinimidyl esters. There are a few variations that have been developed, to overcome some of these issues.

California Red™ SE & SunRed™ SE have much higher labeling efficiency, improved stability, and brighter fluorescence than the corresponding Texas Red® conjugates for long peptides and oligonucleotides. Additionally, Tide Fluor™ 4 has all of these advantages as well as a longer shelf life.

Spectral comparison of California Red™ and Texas Red® conjugated to Gly-Gly-Sor-Ser-Arg-Trp (Red: Texas Red®; Blue: California Red™).
Spectral comparison of California Red™ (Red) and Texas Red® (Blue) conjugated to Gly-Gly-Sor-Ser-Arg-Trp. Comparison made using the AAT Bioquest Fluorescence Spectrum Viewer.

 




 

Featured Products & Resources


AAT Bioquest has a wide range of resources and specialized products for researchers using Texas Red® and similar labels in their work, from Application Notes to FAQs.

Spectrally-similar, succinimidyl ester alternatives to Texas Red®:
California Red™ SE
SunRed™ SE
iFluor™ 594 SE

Customer Favorites
PE-conjugated tandem dyes:
PE-Texas Red Tandem
ReadiUse™ Preactivated PE-iFluor™ 594 Tandem

Online Degree of Labeling (DOL) Calculator:
AAT Bioquest DOL Calculator

HeLa cells were stained with mouse anti-tubulin followed with iFluorTM 594 goat anti-mouse IgG (H+L) (red); actin filaments were stained with Phalloidin-iFluorTM 488 conjugate (green); and nuclei were stained with DAPI (blue).HeLa cells were stained with mouse anti-tubulin followed with iFluor™ 594 goat anti-mouse IgG (H+L) (red); actin filaments were stained with Phalloidin-iFluor™ 488 conjugate (green); and nuclei were stained with DAPI (blue).



 

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