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Investigating Late-Stage Apoptosis With TUNEL Assays

 


Internucleosomal DNA fragmentation caused by activated endonucleases is universally regarded as the biochemical hallmark of apoptosis. Cells containing these DNA strand breaks can be identified with the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay.

AAT Bioquest's Cell Meter™ Assays include all the essential components and a robust protocol to detect and quantitate apoptotic DNA fragmentation in cells. Their excellent performance is achieved by catalytically incorporating a proprietary fluorescent dye modified deoxyuridine 5'-triphosphate nucleotide to the 3' OH ends of DNA using TdT.

Labeled DNA fragments can then be quantitated by flow cytometry or directly visualized by fluorescence microscopy, and the live cell assays can also be used with fluorescent microplate readers.

Apoptotic PCa cells in BM defined by TUNEL staining. PCa cells were recognized by pan cytokeratin with green color, and TUNEL signals were shown with red color. Scale bar, 20 µm. Source: Axl is required for TGF-ß2-induced dormancy of prostate cancer cells in the bone marrow by Yumoto et al., Scientific Reports, Nov. 2016..Featured Citation: Apoptotic PCa cells in BM defined by TUNEL staining. PCa cells were recognized by pan cytokeratin with green color, and TUNEL signals were shown with red color, using the Cell Meter™ Live Cell TUNEL Apoptosis Assay Kit. Source: Axl is required for TGF-ß2-induced dormancy of prostate cancer cells in the bone marrow by Yumoto et al., Scientific Reports (2016).

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TUNEL Assays Digital Catalog

 

Resources & Information


AAT Bioquest has a wide range of resources and specialized products for researchers, from Application Notes, FAQs, and more.

Related Digital Catalogs:
• Annexin-V Staining
• Apoptosis and Necrosis
• Cell Cytotoxicity

AssayWise Newsletter Research Articles:
• A Superior Direct Method for the Quantitative Analysis of DNA Fragments in Late-Stage Apoptosis
• Multiplex Apoptosis and Necrosis

Customer Favorites
Cell Meter™ Kits:
• Live Cell TUNEL Apoptosis Assay Kit *Green Fluorescence*
• Fixed Cell and Tissue TUNEL Apoptosis Assay Kit *Deep Red Fluorescence*

TUNEL assay of formalin-fixed paraffin-embedded (FFPE) human lung adenocarcinoma sections with Cell Meter™ Fixed Cell and Tissue TUNEL Apoptosis Assay Kit *Green Fluorescence*. DNA strand breaks showed intense fluorescent staining in DNAse treated tissue section. The cell nucleus was stained with Nuclear Blue™ DCS1 (Cat No. 17548).TUNEL assay of formalin-fixed paraffin-embedded (FFPE): Human lung adenocarcinoma sections treated with DNAse, with the resultant DNA strand breaks imaged with the Cell Meter™ Fixed Cell and Tissue TUNEL Apoptosis Assay Kit (green). The cell nucleus was stained with Nuclear Blue™ DCS1 (blue).

 

 

 

 

 


 

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