Intracellular Calcium

Measurement: Sensitive Detection

At Any Wavelength

AAT Bioquest has developed the largest collection of calcium indicators, such as our

high-demand Fluo-8®, Cal-520®, and Calbryte™ probes, as well as calcium flux assays

to track calcium mobilization with intense fluorescent signals and in a variety of

wavelength options ranging from ultraviolet to infrared.

Our classic green as well as long-wavelength probes, including our unique NIR calcium

indicator Cal-770™, are manufactured in-house for dependable supply.

Choosing the right probe depends mainly on the experiment. For assistance with product

selection, recommended protocols, or other experimental concerns, contact us.


Long-Wavelength Indicators:

Red-shifted calcium indicators are incredibly useful for researchers looking to perform

multicolor experiments in combination with green- or blue-tagged probes.
Calbryte™ 590 AM and Calbryte™ 630 AM are bright and robust, and can be imaged in

common red channels.
• Like Rhod-2, Rhod-5N AM is essentially nonfluorescent until binding Ca2+, then exhibits

strong fluorescence enhancement with no spectral shift.

Performance Comparison: ATP-stimulated calcium responses

were measured with Rhod-4™ AM and Rhod-2 AM.


Ratiometric Calcium Indicators:


Performance Comparison: ATP-stimulated calcium response

of endogenous P2Y receptor in CHO-K1 cells measured with

Fura-2 AM, Fura-8™ AM and Fura-10™ AM.

Upgraded Single-Wavelength Green Calcium Indicators:

These indicators exhibit significant calcium-dependent changes in fluorescence intensity

at saturating calcium levels without shifting either their absorbance or emission


Cal-520® AM has both high sensitivity and >100 times fluorescence

enhancement when bound to calcium.
• Improved signal/background ratio and intracellular retention properties make

Calbryte™ 520 AM the most robust indicator for evaluating GPCR and calcium

hannel targets
Fluo-8® is a novel, high-performing green calcium indicator which incorporates

the same fluorescein core as Fluo-3 and Fluo-4.

The majority of our indicators are available as membrane-permeant AM esters, and

membrane-impermeant salt derivatives and dextran conjugates. We also offer custom

conjugations as well as stand-alone reagents for researchers with highly unique



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Via Umbria 10
20054 Segrate (Milano)

Tel. +39 02-26922300
Fax +39 02-26926058
Fax +39 02-26923535

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