Intracellular Calcium
Measurement: Sensitive Detection
At Any Wavelength
AAT Bioquest has developed the largest collection of calcium indicators, such as our
high-demand Fluo-8®, Cal-520®, and Calbryte™ probes, as well as calcium flux assays
to track calcium mobilization with intense fluorescent signals and in a variety of
wavelength options ranging from ultraviolet to infrared.
Our classic green as well as long-wavelength probes, including our unique NIR calcium indicator Cal-770™, are manufactured in-house for dependable supply. selection, recommended protocols, or other experimental concerns, contact us.
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Performance Comparison: ATP-stimulated calcium responses
were measured with Rhod-4™ AM and Rhod-2 AM.
Ratiometric Calcium Indicators: |
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Performance Comparison: ATP-stimulated calcium response
of endogenous P2Y receptor in CHO-K1 cells measured with
Fura-2 AM, Fura-8™ AM and Fura-10™ AM.
Upgraded Single-Wavelength Green Calcium Indicators: |
These indicators exhibit significant calcium-dependent changes in fluorescence intensity at saturating calcium levels without shifting either their absorbance or emission wavelengths. |
• Cal-520® AM has both high sensitivity and >100 times fluorescence enhancement when bound to calcium. Calbryte™ 520 AM the most robust indicator for evaluating GPCR and calcium hannel targets |
The majority of our indicators are available as membrane-permeant AM esters, and membrane-impermeant salt derivatives and dextran conjugates. We also offer custom conjugations as well as stand-alone reagents for researchers with highly unique requirements. |
DBA-ITALIA Srl
Via Umbria 10
20054 Segrate (Milano)
Tel. +39 02-26922300
Fax +39 02-26926058
Fax +39 02-26923535