Assay Genie have published an updated version of their 101 Western Blot Troubleshooting Tips Guide!
The Assay Genie Western Blot
Troubleshooting Guide provides comprehensive solutions for common problems
encountered during Western Blot experiments.
It covers issues such as
absent bands, poor transfer, high background, and weak signals.
The guide stresses the
importance of using specific antibodies, proper protein expression and
extraction, correct electrophoresis, transfer techniques, and the use of a
detectable marker on secondary antibodies.
Major sections of the guide include:
Finally, visit Assay
Genie and choose from 1000s of Western blotting validated antibodies.