NoEndo Spin Column Kits for Rapid Endotoxin Removal
NoEndo Spin Columns provide simple and rapid removal of endotoxin from a wide range of protein solutions.
They replace lengthy and expensive chromatographic methods such as phase separation and FPLC.
Using this method, large numbers of samples can be processed at the same time. Additionally, there is negligible hold-up volume;
ensuring high solute recovery with minimal nonspecific absorptive losses.
Protein samples purified using spin columns may be used for a wide range of laboratory procedures such
as biopharmaceutical preparations for proteins, antibodies and vaccines.
Features:
Endotoxin levels as low as 0.05 EU/ml with typical protein recoveries > 90%
Unique flow regulator offers high speed, capture efficiency and reproducibility
SelfSeal membrane technology retains NoEndo resin and sample in the batch incubation chamber
No preparation time or optimisation required
No dilution of the sample
Easy to use pre-packed spin/pump-driven columns
Allow multiple and parallel rapid purifications. This is ideal for rapid scouting, screening and method development
Suitable for a broad spectrum of monoclonal antibodies and proteins
All components of the kits are fully traceable
There are four versions of the Proteus kits; the Proteus NoEndo μ (Micro), the Proteus
NoEndo M (Mini), the Proteus NoEndo S (Standard) and the Proteus NoEndo HC (High
Capacity) spin column kits which are suitable for different endotoxin loads.
HC (High Capacity) For samples with endotoxin loads less than 1,000,000 EU: User Guide
S (Standard) For samples with endotoxin loads less than 30,000 EU User Guide
M (Mini) For samples with endotoxin loads less than 3,000 EU User Guide
µ (Micro) For samples with endotoxin loads between 300-500 EU User Guide
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